OrangerV1, Main, Exploration, bibRecord, 002B19

The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)

Identifieur interne : 002B19 ( Main/Exploration ); précédent : 002B18; suivant : 002B20

The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)

Auteurs : R. L. Boscariol [Brésil] ; W. A. B. Almeida [Brésil] ; M. T. V. C. Derbyshire [Brésil] ; F. A. A. Mourao Filho [Brésil] ; B. M. J. Mendes [Brésil]

Source :

Plant cell reports : (Print) [ 0721-7714 ] ; 2003.

RBID : Pascal:04-0049994

Descripteurs français

Pascal (Inist)
- Mannose-6-phosphate isomerase, Sélection assistée marqueur, Plante transgénique, Multiplication végétative in vitro, Composition chimique, Milieu culture, Comparaison intraspécifique, Cultivar, Citrus sinensis, Agrobacterium tumefaciens, Mannose, Saccharose.
Wicri :
- topic : Plante transgénique, Saccharose.

English descriptors

KwdEn :
- Agrobacterium tumefaciens, Chemical composition, Citrus sinensis, Citrus sinensis (drug effects), Citrus sinensis (genetics), Citrus sinensis (growth & development), Cultivar, Culture Techniques, Culture medium, Fructose (pharmacology), Gene Expression Regulation, Enzymologic (drug effects), Genetic Markers (genetics), Intraspecific comparison, Mannose, Mannose (pharmacology), Mannose-6-Phosphate Isomerase (genetics), Mannose-6-Phosphate Isomerase (metabolism), Mannose-6-phosphate isomerase, Marker assisted selection, Micropropagation, Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Sucrose, Sucrose (pharmacology), Transgenic plant.
MESH :
- chemical , genetics : Genetic Markers, Mannose-6-Phosphate Isomerase.
- chemical , metabolism : Mannose-6-Phosphate Isomerase.
- chemical , pharmacology : Fructose, Mannose, Sucrose.
- drug effects : Citrus sinensis, Gene Expression Regulation, Enzymologic, Plants, Genetically Modified.
- genetics : Citrus sinensis, Plants, Genetically Modified.
- growth & development : Citrus sinensis, Plants, Genetically Modified.
- Culture Techniques.

Abstract

A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.

Affiliations:

Le document en format XML

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<s3>BRA</s3>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Chemical composition</term>
<term>Citrus sinensis</term>
<term>Citrus sinensis (drug effects)</term>
<term>Citrus sinensis (genetics)</term>
<term>Citrus sinensis (growth & development)</term>
<term>Cultivar</term>
<term>Culture Techniques</term>
<term>Culture medium</term>
<term>Fructose (pharmacology)</term>
<term>Gene Expression Regulation, Enzymologic (drug effects)</term>
<term>Genetic Markers (genetics)</term>
<term>Intraspecific comparison</term>
<term>Mannose</term>
<term>Mannose (pharmacology)</term>
<term>Mannose-6-Phosphate Isomerase (genetics)</term>
<term>Mannose-6-Phosphate Isomerase (metabolism)</term>
<term>Mannose-6-phosphate isomerase</term>
<term>Marker assisted selection</term>
<term>Micropropagation</term>
<term>Plants, Genetically Modified (drug effects)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Sucrose</term>
<term>Sucrose (pharmacology)</term>
<term>Transgenic plant</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Genetic Markers</term>
<term>Mannose-6-Phosphate Isomerase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mannose-6-Phosphate Isomerase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Fructose</term>
<term>Mannose</term>
<term>Sucrose</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Citrus sinensis</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus sinensis</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Culture Techniques</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Mannose-6-phosphate isomerase</term>
<term>Sélection assistée marqueur</term>
<term>Plante transgénique</term>
<term>Multiplication végétative in vitro</term>
<term>Composition chimique</term>
<term>Milieu culture</term>
<term>Comparaison intraspécifique</term>
<term>Cultivar</term>
<term>Citrus sinensis</term>
<term>Agrobacterium tumefaciens</term>
<term>Mannose</term>
<term>Saccharose</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term>
<term>Saccharose</term>
</keywords>
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<front><div type="abstract" xml:lang="en">A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
</country>
<region><li>État de São Paulo</li>
</region>
<settlement><li>São Paulo</li>
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<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Boscariol, R L" sort="Boscariol, R L" uniqKey="Boscariol R" first="R. L." last="Boscariol">R. L. Boscariol</name>
</region>
<name sortKey="Almeida, W A B" sort="Almeida, W A B" uniqKey="Almeida W" first="W. A. B." last="Almeida">W. A. B. Almeida</name>
<name sortKey="Derbyshire, M T V C" sort="Derbyshire, M T V C" uniqKey="Derbyshire M" first="M. T. V. C." last="Derbyshire">M. T. V. C. Derbyshire</name>
<name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B. M. J." last="Mendes">B. M. J. Mendes</name>
<name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F. A. A." last="Mourao Filho">F. A. A. Mourao Filho</name>
</country>
</tree>
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</record>

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The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)

The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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